CaspGLOW In Situ Caspase Staining Kits provide simple and sensitive tools for detecting activated caspases in situ in living cells. The assay utilizes potent caspase inhibitors that were conjugated to FITC or rhodamine as the fluorescence in situ markers. The caspase inhibitors are cell permeable, nontoxic, and irreversibly bind to the activated caspases in apoptotic cells. With the FITC or rhodamine labels, activated caspases in apoptotic cells can directly be visualized by fluorescence microscopy, or analyzed by flow cytometry or fluorescence plate reader. Caspase inhibitors without FITC or rhodamine labels were also included in the kits as negative controls.

 
 


 
Detection of Caspase-3 Activation Using CaspGLOWTM Fluorescein Caspase-3 Staining Kit. Apoptosis was induced in Jurkat cells with (B) or without (A) camptothecin for 6 hours. Cells were collected and incubated with CaspGLOWTM in situ marker, FITC-DEVD-FMK, for 20 minutes according to the kit instructions. Results were analyzed by flow cytometry.

 
Product Cat. # Size Data Sheet
CaspGLOW Fluorescein Multi-Caspase Staining Kit K180-25 25 assays Product Data Sheet
CaspGLOW Fluorescein Multi-Caspase Staining Kit K180-100 100 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-2 Staigning Kit K182-25 25 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-2 Staigning Kit K182-100 100 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-3 Staining Kit K183-25 25 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-3 Staining Kit K183-100 100 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-8 Staining Kit K188-25 25 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-8 Staining Kit K188-100 100 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-9 Staining Kit K189-25 25 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-9 Staining Kit K189-100 100 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-12 Staining Kit K172-25 25 assays Product Data Sheet
CaspGLOW Fluorescein Caspase-12 Staining Kit K172-100 100 assays Product Data Sheet
CaspGLOW Red Multi-Caspase Staining Kit K190-25 25 assays Product Data Sheet
CaspGLOW Red Multi-Caspase Staining Kit K190-100 100 assays Product Data Sheet
CaspGLOW Red Caspase-3 Staining Kit K193-25 25 assays Product Data Sheet
CaspGLOW Red Caspase-3 Staining Kit K193-100 100 assays Product Data Sheet
CaspGLOW Red Caspase-8 Staining Kit K198-25 25 assays Product Data Sheet
CaspGLOW Red Caspase-8 Staining Kit K198-100 100 assays Product Data Sheet
CaspGLOW Red Caspase-9 Staining Kit K199-25 25 assays Product Data Sheet
CaspGLOW Red Caspase-9 Staining Kit K199-100 100 assays Product Data Sheet

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