Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases has been shown to delay apoptosis, implicating a potential role in drug screening efforts. We offer a full line of Caspase Inhibitor Drug Screening Kits for effective screening of caspase inhibitors using fluorometric methods. The assays utilize peptide substrates consisting of the consensus cleavage sequence for each caspase, labeled with AFC (7-amino-4-trifluoromethyl coumarin). Active caspases (provided in the kits) cleave the synthetic substrates to release free AFC which can then be quantified using a microtiter plate reader. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspases can then be determined. The assays are simple, straightforward, and can be performed directly in microtiter plates. Assay conditions for each caspase have been optimized to obtain the maximal activity. Each kit contains 100 units of active caspases, sufficient for screening 100 samples.

Inhibition of Caspase Activity by Caspase Inhibitors. Caspase assays were performed using 1 unit of active caspase-3 (A) or 8 (B) in the presence and absence of caspase-3 (Z-DEVD-FMK) or -8 (Z-IETD-FMK) inhibitor as indicated. Caspase-3 and -8 activities were analyzed in 96-well plate by fluorescence plate reader.

Products Cat.# Size Data Sheet
Caspase-1 Inhibitor Drug Screening Kit K151-100 100 assays Product Data Sheet
Caspase-2 Inhibitor Drug Screening Kit K152-100 100 assays Product Data Sheet
Caspase-3 Inhibitor Drug Screening Kit K153-100 100 assays Product Data Sheet
Caspase-4 Inhibitor Drug Screening Kit K154-100 100 assays Product Data Sheet
Caspase-5 Inhibitor Drug Screening Kit K155-100 100 assays Product Data Sheet
Caspase-6 Inhibitor Drug Screening Kit K156-100 100 assays Product Data Sheet
Caspase-7 Inhibitor Drug Screening Kit K157-100 100 assays Product Data Sheet
Caspase-8 Inhibitor Drug Screening Kit K158-100 100 assays Product Data Sheet
Caspase-9 Inhibitor Drug Screening Kit K159-100 100 assays Product Data Sheet
Caspase-10 Inhibitor Drug Screening Kit K160-100 100 assays Product Data Sheet