Caspase-3 and -7 share the same target substrate sequence (DEVD) and thus it is difficult to differentiate the cleavage activity attributed by these two caspases in vitro. The CaspSELECTTM Caspase-3 & -7 Immunoassay Kits allow quantification of the specific activity of caspase-3 and -7, respectively. The assay utilizes caspase-3 or -7 specific antibody to capture the activated caspase-3 or -7 in cell lysate. Specific activity of caspase-3 or -7 can then be analyzed using the common caspase-3/7 substrate DEVD-AFC. The assay system ensures detection of only caspase-3 or caspase-7 specific activity in apoptotic samples. Other caspases and non-specific proteases are not detected.

Time-Dependent Induction of Caspase-3 Specific Activity Analyzed by CaspSELECTTM Caspase-3 Immunoassay Kit. Apoptosis was induced by camptothecin (2 mM) for various hours as indicated. Caspase-3-specific activity was analyzed using CaspSELECTTM Caspase-3 Immunoassay Kit according to the kit instructions.

Product Cat. # Size Data Sheet
CaspSCREENTM Caspase-3 Immunoassay Kit K163-100 100 assays Product Data Sheet
CaspSCREENTM Caspase-7 Immunoassay Kit K167-100 100 assays Product Data Sheet