GENTAUR Hybridoma Growth Supplement

For Serum-Free Hybridoma Growth Medium

GENTAUR is pleased to inform you we have a replacement for the FC210001 ORIGEN
Hybridoma Cloning factor.Our alternative product enables you to easily prepare your
own Serum-Free Hybridoma Growth Medium.


The TX-HYB Hybridoma Growth Supplement replaces feeder layers or other conditioned media and growth factors used in hybridoma production. TX-HYB increases the yield of hybridomas surviving HAT selection, the cloning efficiency of B-cell hybridomas, and the number of antibody producing colonies.


The Supplement is ready to use. Prepare the serum free Hybridoma growth medium by dissolving 1 bottle of TX-HYB (11 ml) into 1 Liter of D-MEM F-12 (Dulbecco's Modified Eagle's Medium Ham’s F-12). We recommend product L0093 from Biosera, see below.


The prepared Serum-Free Hybridoma growth medium is a chemically defined culture medium supporting the serum-free growth of various hybridomas and 293 cells. The compounds of Hybridoma growth medium include water for injection, a modified DMEM/F12 base, HEPES buffer, known amounts of insulin, transferin, testosterone, sodium selenite, ethanolamine, a variety of saturated and unsaturated fatty acids, and stabilizing proteins. TX-HYB Hybridoma growth supplement contains no bovine serum albumine or other undefined protein mixtures.


The TX-HYB Hybridoma Growth Supplement can be used until 6 months after manufacture, when properly stored at 4°C.

The expiry date is on the bottle.


The prepared Hybridoma growth medium can be stored at 2°C – 8°C for at least 3 months


 Dulbecco's Modified Eagle's Medium – Ham’s F-12


The DMEM – Ham’s F12 is a serum-free medium formulation for general use. This formulation is a 1:1 blend of DMEM and Ham's F12 media supplied complete, ready to use with L-glutamin, Hepes buffer and pyridoxine HCl for culturing a wide range of cell types including Hybridoma cells in combination with Gentaur’s TX-HYB Hybridoma Growth Supplement.


L0093 DMEM – Ham’s F12 with L-Glutamine with 15mM HEPES 500 ml


Use the TX-HYB supplement together with the

DMEM F-12 medium to support optimal hybridoma

development and achieve an optimal cell density!

Code: L0093
N° of Bottles Price
5x500 ml 59
10x500 ml 99
20x500 ml 169


Code: TX-HYB
N° of Bottles Price
1x11 ml 57€
5x11 ml 270€
10x11 ml 510€

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