PARP Pharmacodynamic Assay

PARP catalyzes the NAD-dependent addition of poly (ADP-ribose) (PAR) onto itself and adjacent nuclear proteins. Furthermore, polymorphisms in the PARP-1 gene correspond to disease predisposition, and variation in PARP activity might have an impact on the effects of PARP inhibitors in clinical settings. To address the need to monitor PARP activity among different individuals, and within cells, validated PARP in vivo Pharmacodynamic Assay measures net PAR levels in cellular extracts and has been used to document differences in PAR levels among tumor lysates from different tissues, organs and xenografts.

 Features

     Chemiluminescent, non-radioactive format

     Higher throughput, 96 test size

     Sensitivity down to 10 pg/ml of PAR

 Applications

     Quantification of PAR in peripheral blood mononuclear cells and tissue culture cells

     Monitoring the efficacy of PARP inhibitors on cellular PAR formation

     Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination

      therapy.

 

Code Product Name Quantity Price
4510-096-K PARP in vivo Pharmacodynamic Assay 1x 96 well 1269
4690-096-K HT Fluorescent Homogen. PARP Inhibition Assay 1x 96 well 489
4684-096-K HT Colorimetric PARP/Apoptosis Assay 1x 96 well 533
4685-096-K HT Chemiluminescent PARP/Apoptosis Assay 1x 96 well 533

Centrifuge Tubes

 

    RACKED

    Sterile

Text Box: 50 mL & 15 mL

 

    Non-Pyrogenic

    Polypropylene

    Flat-Top, Screw-on Caps

    Conical Bottoms

    White, Easy to Read, Printed Graduations

    White Writing Area, for Easy Sample Identification

    0C to 40C

    Bulk Packed per 500, racked per 25  

    Maximum Recommended RCF Rating (50mL): 9400 xg

    Maximum Recommended RCF Rating (15mL): 8400 xg

 

Code Product Name Quantity Price
oct-51 15ml Centrifuge Tubes, Sterile,Flat-top cap 500 47
oct-01 50ml Centrifuge Tubes, Sterile,Flat-top cap 500 62

 

Protein Extraction Tubes

 

The GeBAflex-tubes are the most efficient, user- friendly dialysis & buffer exchange tool in the market today.
Experience the comfort of standard pipette loading and unloading, wide range of volumes & MWCO and a 98% sample recovery yield, all at affordable prices.

Owing to its revolutionary design the GeBAflex-tube is the only dialysis tool available which allows for true high throughput dialysis, accommodating a large number of samples in a single buffer tank.

The GeBAflex-tube allows rapid, high performance extraction of macromolecules without any contamination and is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.

 Applications

    Dialysis or buffer exchange of volumes between 10ml-20ml.

    Preparation of protein samples for MALDI-MS.

    Sample concentration.

    Large-scale protein dialysis such as antibodies and recombinant protein purification.

    Peptide dialysis small as 10 amino acids.

    Virus-particle purification.

    Removal of contaminating micro molecules.

    Tissue culture extracts purification.

    Removal of salts, surfactants, solvents and detergents.

    Complex formation studies (protein-protein, protein-DNA and protein-RNA).

    pH and buffer adjustment of sample solution, protein extract or cell extract.

    High through put dialysis, a single beaker for several GeBAflex-tubes on one floating pad

 

Comes in 4 Sizes:

    Mini: 10-250l

    Midi: 50-800l

    Maxi: 0.1-3ml

    Maxi: 0.1-3ml

    Mega: 3-20ml

Available MWCO:

    1000

    3500

    6000-8000

    12000-14000

    25000

    50000

 

 

Code Product Name Qty. Price
T010 Midi kit, 3500 cut-off 10 91
T012 Midi kit, 3500 cut-off 30 152
T021 Midi kit, 6000-8000 cut-off 15 105
T055 Maxi kit, 12000-14000 cut-off 15 102
D010 Midi kit, 3500 cut-off 10 70
D020 Midi kit, 6000-8000 cut-off 10 70
D022 Midi kit, 6000-8000 cut-off 30 112
D030 Maxi kit, 3500 cut-off 5 61
D045 Maxi kit, 6000-8000 cut-off 15 91
D055 Maxi kit, 12000-14000 cut-off 15 91
D080-6 Mega kit, 6000-8000 cut-off 10 43

More information on www.gentaur.com

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